Publication: Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development
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Date
2013
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Publisher
Elsevier Science Inc
Abstract
The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using sera from typhoid patients showed the presence of HlyE-specific antibodies in circulation. (c) 2013 Elsevier Inc. All rights reserved.
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Keywords
Salmonella, Typhi, HlyE, Antigen, Immunoblot, ELISA