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Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: Potential application for immunoassay development

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Cloning, expression, and purification of the hemolysin,cytolysin HlyE antigen from Salmonella enterica serovar Typhi_ Potential application for immunoassay development.pdf (457.58 KB)

Date

2013

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Elsevier Inc.

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Abstract

The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using sera from typhoid patients showed the presence of HlyE-specific antibodies in circulation. � 2013 Elsevier Inc.

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Antigen, HlyE, Immunoblot, ELISA, Salmonella, Typhi

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https://www.scopus.com/inward/record.uri?eid=2-s2.0-84881544694&doi=10.1016%2fj.diagmicrobio.2013.05.010&partnerID=40&md5=6c9793de23d716da35ad8743cd9e0f66
 https://oarep.usim.edu.my/handle/123456789/8921

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